Publications

2024

Danlasky, B.M., Martinez, M., Cassani, M., Bodas, D., Putnam, A., and Seydoux, G. (2024). The P granule antibody KT3 recognizes epitopes in both PGL-1 and PGL-3. MicroPublication Biol. 10.17912/micropub.biology.001137. PMCID: PMC10910292

2021

Folkmann AW, Putnam A, Lee CF, Seydoux G. Regulation of biomolecular condensates by interfacial protein clusters. Science. 2021 Sep 10;373(6560):1218-1224. doi: 10.1126/science.abg7071. Epub 2021 Sep 9. PubMed PMID: 34516789; PubMed Central PMCID: PMC8627561.

Gao J, Gao Z, Putnam AA, Byrd AK, Venus SL, Marecki JC, Edwards AD, Lowe HM, Jankowsky E, Raney KD. G-quadruplex DNA inhibits unwinding activity but promotes liquid-liquid phase separation by the DEAD-box helicase Ded1p. Chem Commun (Camb). 2021 Jul 27;57(60):7445-7448. doi: 10.1039/d1cc01479j. PubMed PMID: 34232232; PubMed Central PMCID: PMC8315639.

Schmidt H, Putnam A, Rasoloson D, Seydoux G. Protein-based condensation mechanisms drive the assembly of RNA-rich P granules. Elife. 2021 Jun 9;10. doi: 10.7554/eLife.63698. PubMed PMID: 34106046; PubMed Central PMCID: PMC8238508.

2020

Babinchak WM, Dumm BK, Venus S, Boyko S, Putnam AA, Jankowsky E, Surewicz WK. Small molecules as potent biphasic modulators of protein liquid-liquid phase separation. Nat Commun. 2020 Nov 4;11(1):5574. doi: 10.1038/s41467-020-19211-z. PubMed PMID: 33149109; PubMed Central PMCID: PMC7643064.

Naineni SK, Itoua Maïga R, Cencic R, Putnam AA, Amador LA, Rodriguez AD, Jankowsky E, Pelletier J. A comparative study of small molecules targeting eIF4A. RNA. 2020 May;26(5):541-549. doi: 10.1261/rna.072884.119. Epub 2020 Feb 3. PubMed PMID: 32014999; PubMed Central PMCID: PMC7161356.

Lee CS, Putnam A, Lu T, He S, Ouyang JPT, Seydoux G. Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins. Elife. 2020 Jan 24;9. doi: 10.7554/eLife.52896. PubMed PMID: 31975687; PubMed Central PMCID: PMC7007223.

2019

Gao J, Byrd AK, Zybailov BL, Marecki JC, Guderyon MJ, Edwards AD, Chib S, West KL, Waldrip ZJ, Mackintosh SG, Gao Z, Putnam AA, Jankowsky E, Raney KD. DEAD-box RNA helicases Dbp2, Ded1 and Mss116 bind to G-quadruplex nucleic acids and destabilize G-quadruplex RNA. Chem Commun (Camb). 2019 Apr 11;55(31):4467-4470. doi: 10.1039/c8cc10091h. PubMed PMID: 30855040; PubMed Central PMCID: PMC6459694.

Putnam A, Cassani M, Smith J, Seydoux G. A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos. Nat Struct Mol Biol. 2019 Mar;26(3):220-226. doi: 10.1038/s41594-019-0193-2. Epub 2019 Mar 4. PubMed PMID: 30833787; PubMed Central PMCID: PMC6668929.

2017

Sharma D, Putnam AA, Jankowsky E. Biochemical Differences and Similarities between the DEAD-Box Helicase Orthologs DDX3X and Ded1p. J Mol Biol. 2017 Nov 24;429(23):3730-3742. doi: 10.1016/j.jmb.2017.10.008. Epub 2017 Oct 13. PubMed PMID: 29037760; PubMed Central PMCID: PMC5693625.

Pre-2016

Gao Z, Putnam AA, Bowers HA, Guenther UP, Ye X, Kindsfather A, Hilliker AK, Jankowsky E. Coupling between the DEAD-box RNA helicases Ded1p and eIF4A. Elife. 2016 Aug 5;5. doi: 10.7554/eLife.16408. PubMed PMID: 27494274; PubMed Central PMCID: PMC4990422.

Putnam AA, Gao Z, Liu F, Jia H, Yang Q, Jankowsky E. Division of Labor in an Oligomer of the DEAD-Box RNA Helicase Ded1p. Mol Cell. 2015 Aug 20;59(4):541-52. doi: 10.1016/j.molcel.2015.06.030. Epub 2015 Jul 23. PubMed PMID: 26212457; PubMed Central PMCID: PMC4546514.

Saikia M, Jobava R, Parisien M, Putnam A, Krokowski D, Gao XH, Guan BJ, Yuan Y, Jankowsky E, Feng Z, Hu GF, Pusztai-Carey M, Gorla M, Sepuri NB, Pan T, Hatzoglou M. Angiogenin-cleaved tRNA halves interact with cytochrome c, protecting cells from apoptosis during osmotic stress. Mol Cell Biol. 2014 Jul;34(13):2450-63. doi: 10.1128/MCB.00136-14. Epub 2014 Apr 21. PubMed PMID: 24752898; PubMed Central PMCID: PMC4054315.

Liu F, Putnam AA, Jankowsky E. DEAD-box helicases form nucleotide-dependent, long-lived complexes with RNA. Biochemistry. 2014 Jan 21;53(2):423-33. doi: 10.1021/bi401540q. Epub 2014 Jan 7. PubMed PMID: 24367975.

Putnam AA, Jankowsky E. AMP sensing by DEAD-box RNA helicases. J Mol Biol. 2013 Oct 23;425(20):3839-45. doi: 10.1016/j.jmb.2013.05.006. Epub 2013 May 20. PubMed PMID: 23702290; PubMed Central PMCID: PMC3795843.

Saguez C, Gonzales FA, Schmid M, Bøggild A, Latrick CM, Malagon F, Putnam A, Sanderson L, Jankowsky E, Brodersen DE, Jensen TH. Mutational analysis of the yeast RNA helicase Sub2p reveals conserved domains required for growth, mRNA export, and genomic stability. RNA. 2013 Oct;19(10):1363-71. doi: 10.1261/rna.040048.113. Epub 2013 Aug 20. PubMed PMID: 23962665; PubMed Central PMCID: PMC3854527.

Nayak NR, Putnam AA, Addepalli B, Lowenson JD, Chen T, Jankowsky E, Perry SE, Dinkins RD, Limbach PA, Clarke SG, Downie AB. An Arabidopsis ATP-dependent, DEAD-box RNA helicase loses activity upon IsoAsp formation but is restored by PROTEIN ISOASPARTYL METHYLTRANSFERASE. Plant Cell. 2013 Jul;25(7):2573-86. doi: 10.1105/tpc.113.113456. Epub 2013 Jul 31. PubMed PMID: 23903319; PubMed Central PMCID: PMC3753384.

Liu F, Putnam A, Jankowsky E. ATP hydrolysis is required for DEAD-box protein recycling but not for duplex unwinding. Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20209-14. doi: 10.1073/pnas.0811115106. Epub 2008 Dec 16. PubMed PMID: 19088201; PubMed Central PMCID: PMC2629341.